In Situ Cell Death Detection Kit, Fluorescein

TUNEL assay cell type - SK-MEL-2 human melanoma

Experiment
TUNEL assay cell type - SK-MEL-2 human melanoma
Product
In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- Cells were fixed with 4% paraformaldehyde for 20 min.

- Permeabilized with PBS containing 0.1% Triton X-100 and 0.1% glycine for 2 min on ice
Downstream tips
- When Nonspecific labelling is present, reduce concentration of TdT.

- When high background is present reduce concentration of labelling mix by 50%

Publication protocol

Sk-mel-2 cells mounted on glass slides were fixed with 4% paraformaldehyde for 20 min, and permeabilized with PBS containing 0.1% Triton X-100 and 0.1% glycine for 2 min on ice. The TUNEL staining was performed using the In Situ Cell Death Detection Kit, Fluorescein (Roche Diagnostics, Germany). Cells were co-stained with 4′6′-diamidino-2-phenylindole (DAPI) to visualize nuclei. The images were taken using a FSX100 all-in-one microscope (Olympus Corporation, Japan).

Full paper   Login or join for free to view the full paper.

Reviews

In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - SK-MEL-2 human melanoma using In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich.

Paper title
α-Mangostin, a Natural Agent, Enhances the Response of NRAS Mutant Melanoma to Retinoic Acid
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, Fluorescein below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - SK-MEL-2 human melanoma using In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms